Composite

Part:BBa_K2314115:Design

Designed by: Yuqing Chen   Group: iGEM17_OUC-China   (2017-10-22)


Ice nuclear protein and monomer streptavidin fusion protein(INP-MSA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 426
    Illegal AgeI site found at 1120
    Illegal AgeI site found at 1180
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Between INP and mSA there is a 4X linker which ensure mSA can express correctly.


Source

INP is a part of the composed part K523013 which designed by Edinburg in iGEM part registry. J23106 is member of constitutive family in iGEM part registry. And mSA is provided kindly by Peking University.

Van, Bloois E, et al. "Decorating microbes: surface display of proteins on Escherichia coli." Trends in Biotechnology 29.2(2011):79. Demonte, Daniel, et al. "Structure‐based engineering of streptavidin monomer with a reduced biotin dissociation rate." Proteins Structure 81.9(2013):1621-33.

References