Composite
Part:BBa_K2314115:Design
Designed by: Yuqing Chen Group: iGEM17_OUC-China (2017-10-22)
Ice nuclear protein and monomer streptavidin fusion protein(INP-MSA)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 426
Illegal AgeI site found at 1120
Illegal AgeI site found at 1180 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Between INP and mSA there is a 4X linker which ensure mSA can express correctly.
Source
INP is a part of the composed part K523013 which designed by Edinburg in iGEM part registry. J23106 is member of constitutive family in iGEM part registry. And mSA is provided kindly by Peking University.
Van, Bloois E, et al. "Decorating microbes: surface display of proteins on Escherichia coli." Trends in Biotechnology 29.2(2011):79. Demonte, Daniel, et al. "Structure‐based engineering of streptavidin monomer with a reduced biotin dissociation rate." Proteins Structure 81.9(2013):1621-33.